Mario Del Rosario is a postdoctoral researcher in the Henriques Lab at ITQB NOVA, specializing in expansion microscopy and advanced biological imaging techniques. His research focuses on developing innovative methods to visualize biological structures at nanoscale resolution through physical expansion techniques. As a workshop instructor for the Expansion Microscopy workshop, Mario will guide participants through theoretical foundations, hands-on sample preparation protocols, imaging strategies, and data analysis techniques for successful expansion microscopy experiments.